The Ever Changing Coronavirus Tests


wisp

This is a guide map to COVID testing confusion, the test now is not the same as the test we started with in 2020.

The PCR test for COVID was supposed to have been taken off the market at the end of 2021 as it was only for emergency use and never approved as a real test.

‘After December 31, 2021, CDC will withdraw the request to the U.S. Food and Drug Administration (FDA) for Emergency Use Authorization (EUA) of the CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel, the assay first introduced in February 2020 for detection of SARS-CoV-2 only. CDC is providing this advance notice for clinical laboratories to have adequate time to select and implement one of the many FDA-authorized alternatives.’

07/21/2021: Lab Alert: Changes to CDC RT-PCR for SARS-CoV-2 Testing

https://www.cdc.gov/csels/dls/locs/2021/07-21-2021-lab-alert-Changes_CDC_RT-PCR_SARS-CoV-2_Testing_1.html

So along came Omicron which was defined as positive in customers and patients by taking off one of the primers from original PCR test which is called the S gene or the spike protein gene. This change made an increase in cases inevitable and allowed the original test continued use and sales.

‘The number of cases of this variant appears to be increasing in almost all provinces in South Africa. Current SARS-CoV-2 PCR diagnostics continue to detect this variant. Several labs have indicated that for one widely used PCR test, one of the three target genes is not detected (called S gene dropout or S gene target failure) and this test can therefore be used as marker for this variant, pending sequencing confirmation. Using this approach, this variant has been detected at faster rates than previous surges in infection, suggesting that this variant may have a growth advantage.’

Classification of Omicron (B.1.1.529): SARS-CoV-2 Variant of Concern

https://www.who.int/news/item/26-11-2021-classification-of-omicron-(b.1.1.529)-sars-cov-2-variant-of-concern

Remember the mRNA gene therapy vaccine is supposed to create spike proteins from your DNA so you make antibodies to the spike protein.

And Omicron does not have the spike protein? 

Meaning Omicron is not COVID-19 and the vaccines intended to make antibodies to COVID-19 spike proteins are completely useless.

‘The five genomic regions of the Coronavirus SARS-2 virus including Nucleocapsids (N), Envelope (E), RNA depended RNA Polymerase (RdRp), ORF1ab and Spike (S) were selected for primer designing.’

Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7340604/

These are the 3 primers of the original PCR test.

‘The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability.

The reliability of the test is also dependent on the reagents used to allow the enzyme to amplify and detect its target. These are the SARS-CoV-2-specific primers and probe, which must be 100% specific for the virus and so amplify only viral sequences and report the increasing amount of PCR amplicon being synthesised. There are several viral genomic regions targeted by RT-qPCR assays, and multiple primer designs targeting the same genes. These include the RdRP gene (RNA-dependent RNA polymerase gene) (Nsp12), the E gene (envelope protein gene), and the N gene (nucleocapsid protein gene). 

RT-qPCR Testing of SARS-CoV2: A Primer

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7215906/

These are the same genes from before 2020 in coronaviruses.

‘The coronavirus E protein is a small membrane protein that has an important role in the assembly of virions.’ 2012

The Coronavirus E Protein: Assembly and Beyond

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347032/

‘The coronavirus nucleocapsid (N) is a structural protein that forms complexes with genomic RNA, interacts with the viral membrane protein during virion assembly and plays a critical role in enhancing the efficiency of virus transcription and assembly.’ 2014

The coronavirus nucleocapsid is a multifunctional protein

https://pubmed.ncbi.nlm.nih.gov/25105276/

‘The SARS (severe acute respiratory syndrome) pandemic caused ten years ago by the SARS-coronavirus (SARS-CoV) has stimulated a number of studies on the molecular biology of coronaviruses. This research has provided significant new insight into many mechanisms used by the coronavirus replication-transcription complex (RTC). The RTC directs and coordinates processes in order to replicate and transcribe the coronavirus genome, a single-stranded, positive-sense RNA of outstanding length (∼27–32 kilobases). Here, we review the up-to-date knowledge on SARS-CoV replicative enzymes encoded in the ORF1b, i.e., the main RNA-dependent RNA polymerase (nsp12), the helicase/triphosphatase (nsp13), two unusual ribonucleases (nsp14, nsp15) and RNA-cap methyltransferases (nsp14, nsp16). We also review how these enzymes co-operate with other viral co-factors (nsp7, nsp8, and nsp10) to regulate their activity.’ 2012

SARS-CoV ORF1b-encoded nonstructural proteins 12–16: Replicative enzymes as antiviral targets

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7113864/

All of it has been non-specific.

1.   The genetic strand of SARS-CoV-2 is only one model, which was created by an alignment. In the publication of Fan Wu et al, in Nature, Vol 579 of 3.2.2020 (16), in which the genome (complete genetic strand) of SARS-CoV-2 was presented for the first time, it became the template for all further alignments of all other virologists and biochemists.

2.   The genome (genetic strand) of SARS-CoV-2 has never been isolated, only the entire RNA obtained from a bronchial lavage (BALF) of a patient has been used.

3.   No control experiments were performed to exclude that the gene sequences are tissue-own structures.

Corona: The comprehensible and verifiable refutation of the virus allegations

https://telegra.ph/corona-the-comprehensible-and-verifiable-refutation-of-the-virus-allegations-10-29

In January 2021 they turned down the cycles of the PCR test from 45 to 25 which should have reduced case numbers so the test in 2021 was not the same test as in 2020.

‘The World Health Organization (WHO) itself has admitted that the entire basis for collating “case” numbers since the beginning of this ‘global pandemic’ is effectively null and void. In its directive published in late January, the organization stated that medical professionals should not be using PCR Testing with high Cycle Threshold (CT) levels due to the high likelihood of generating false positives in people, and also that the PCR Test should not be used as the sole metric for diagnosing and should be accompanied by a professional clinical diagnosis. In other words: the PCR Test cannot rightly be used as a medical diagnostic tool, and yet, it has been widely used as such for the last 12 months. This admission should have grave implications for every public health official, politician and media editor on the planet, but the silence is deafening – as most are simply ignoring this fact.’

Dr. Scott Jensen, WHO Confirm: ‘We’ve All Been Played’ on COVID-19

https://21stcenturywire.com/2021/03/07/dr-scott-jensen-who-confirm-weve-all-been-played-on-covid-19/

So which coronavirus are we testing for?

‘Coronavirus. These tend to do their dirty work in the winter and early spring. The coronavirus is the cause of about 20% of colds. There are more than 30 kinds, but only three or four affect people.

RSV and parainfluenza. These viruses cause 20% of colds. They sometimes lead to severe infections, like pneumonia, in young children.’

Causes of the Common Cold

https://www.webmd.com/cold-and-flu/cold-guide/common_cold_causes

Remember, don’t confuse common colds with COVID-19.

‘Common human coronaviruses, including types 229E, NL63, OC43, and HKU1, usually cause mild to moderate upper-respiratory tract illnesses, like the common cold. Most people get infected with one or more of these viruses at some point in their lives. This information applies to common human coronaviruses and should not be confused with coronavirus disease 2019 (formerly referred to as 2019 Novel Coronavirus).

Symptoms of common human coronaviruses:

·         runny nose

·         sore throat

·         headache

·         fever

·         cough

·         general feeling of being unwell

Human coronaviruses can sometimes cause lower-respiratory tract illnesses, such as pneumonia or bronchitis. This is more common in people with cardiopulmonary disease, people with weakened immune systems, infants, and older adults.

symptom icon

Transmission of common human coronaviruses

Common human coronaviruses usually spread from an infected person to others through

·         the air by coughing and sneezing

·         close personal contact, like touching or shaking hands

·         touching an object or surface with the virus on it, then touching your mouth, nose, or eyes before washing your hands

In the United States, people usually get infected with common human coronaviruses in the fall and winter, but you can get infected at any time of the year. Young children are most likely to get infected, but people can have multiple infections in their lifetime.

Common Human Coronaviruses

https://www.cdc.gov/coronavirus/general-information.html

The RAT kits as they are called now are as worthless as the PCR tests.

‘The U.S. Food and Drug Administration (FDA) is alerting clinical laboratory staff and health care providers that false positive results can occur with antigen tests, including when users do not follow the instructions for use of antigen tests for the rapid detection of SARS-CoV-2. Generally, antigen tests are indicated for the qualitative detection of SARS-CoV-2 antigens in authorized specimen types collected from individuals who are suspected of COVID-19 by their healthcare provider within a certain number of days of symptom onset. The FDA is aware of reports of false positive results associated with antigen tests used in nursing homes and other settings and continues to monitor and evaluate these reports and other available information about device safety and performance.’

Potential for False Positive Results with Antigen Tests for Rapid Detection of SARS-CoV-2 – Letter to Clinical Laboratory Staff and Health Care Providers

https://www.fda.gov/medical-devices/letters-health-care-providers/potential-false-positive-results-antigen-tests-rapid-detection-sars-cov-2-letter-clinical-laboratory

If anyone wants to claim they can see COVID under the microscope so all of this is anti-science, check what the kidney doctors say about that.

‘Since the discovery of the causative agent for the novel severe acute respiratory syndrome (SARS)–like pneumonia syndrome pandemic that started in China in 2019 (1,2), a coronavirus named SARS coronavirus 2 (SARS-CoV-2), electron microscopy images have populated the medical literature (2) and media outlets alike displaying the characteristic 60–140 nm round particles surrounded by a “corona” of 9–12 nm distinctive spikes (2). Although many of these images were obtained after “in vitro” infection of cultured cells with SARS-CoV-2 (2) and are thus likely a true representation of viral particles, we have observed morphologically indistinguishable inclusions within podocytes and tubular epithelial cells both in patients negative for coronavirus disease 2019 (COVID-19) as well as in renal biopsies from the pre–COVID-19 era. Although direct infection of the kidney is theoretically possible, given the presence of angiotensin-converting enzyme 2 (the receptor used by SARS-CoV-2 to gain access to cells) within proximal tubular epithelium (3) and podocytes (4), the virus has not been detected by real-time RT-PCR in urine samples from patients with COVID19 (5–7). Additionally, for the virus to have access to kidney parenchyma, viremia should occur, and this has only been detected in a minority of patients (6–8). We would, therefore, like to issue a note of caution for inferring viral tissue infection by morphology alone using electron microscopy images from tissues obtained from biopsies or autopsy material in patients with COVID-19.’

Appearances Can Be Deceiving – Viral-like Inclusions in COVID-19 Negative Renal Biopsies by Electron Microscopy

https://kidney360.asnjournals.org/content/kidney360/1/8/824.full.pdf

In fact, the only people who seem skeptical and analytical about the validity of the COVID tests are health professionals, and unfortunately they are being shouted down by media and a frenzied public.

‘Dear Editor

We are told that the virus is everywhere – in the air, in our breath, on fomites, trapped in masks – yet public health authorities seem not to be in possession of any cultivable clinical samples of the offending pathogen.

In March 2020, the World Health Organisation instructed authorities not to look for a virus but to rely instead on a genome test, the RT-PCR, which is not specific for SARS-CoV-2 (1) (2).

A Freedom of Information request to Public Health England about cultivable clinical samples or direct evidence of viral isolation has no information and refers to the proxy RT-PCR test, quoting Eurosurveillance (3).

Eurosurveillance states: “Virus detection by reverse transcription-PCR (RT-PCR) from respiratory samples is widely used to diagnose and monitor SARS-CoV-2 infection and, increasingly, to infer infectivity of an individual. However, RT-PCR does not distinguish between infectious and non-infectious virus. Propagating virus from clinical samples confirms the presence of infectious virus but is not widely available (and) requires biosafety level 3 facilities” (4).

The CDC admits that, “no quantified virus isolates of the 2019-nCoV are currently available”, and used a genetically modified human lung alveolar adenocarcinoma cell culture to, “mimic clinical specimen”(5).

It appears, therefore, that we have public health bodies without clinical samples, a test which is non-specific and does not distinguish between infectivity and non-infectivity, a requirement for biosafety level 3 facilities to even look for a virus, yet we are led to believe that it is up all our noses.

So, where is the virus?

https://www.bmj.com/content/370/bmj.m3379/rr-2

‘The published RT-qPCR protocol for detection and diagnostics of 2019-nCoV and the manuscript suffer from numerous technical and scientific errors, including insufficient primer design, a problematic and insufficient RT-qPCR protocol, and the absence of an accurate test validation. Neither the presented test nor the manuscript itself fulfils the requirements for an acceptable scientific publication. Further, serious conflicts of interest of the authors are not mentioned. Finally, the very short timescale between submission and acceptance of the publication (24 hours) signifies that a systematic peer review process was either not performed here, or of problematic poor quality.  We provide compelling evidence of several scientific inadequacies, errors and flaw’

External peer review of the RTPCR test to detect SARS-CoV-2 reveals 10 major scientific flaws at the molecular and methodological level: consequences for false positive results

https://www.researchgate.net/publication/346483715_External_peer_review_of_the_RTPCR_test_to_detect_SARS-CoV-2_reveals_10_major_scientific_flaws_at_the_molecular_and_methodological_level_consequences_for_false_positive_results

https://cormandrostenreview.com/report/

‘The number of people dying today is the same as it would be any other year’, (17 November 2020). Instead, excess total deaths have been driven by lack of treatment due to hospital closure/lockdowns and have occurred mostly at home.’ Professor Brookes, a Health Data Scientist from the University of Leicester’

‘Hijacking of science by vested interests has resulted in immeasurable harms to society. Lockdowns, meant to save lives but being pushed by narratives that have little basis in science, have themselves caused loss of life, livelihoods, dignity, and humanity. We need to ask how we have got to this sorry state.’ 

Covid-19: politicisation, “corruption,” and suppression of science

https://www.bmj.com/content/371/bmj.m4425/rr-31

Or in Australia where doctors have to hide their identity if they want to blow the whistle.

Australian doctor blowing whistle 15.12.2021

On June 23rd 2022 sacked Australian doctors spent 3 and a half hours in the QLD parliament exposing how much we have been lied to to sell COVID.

AMPS Medico-Legal Parliamentary Summit

The National Law and Therapeutic Goods Act have precipitated disastrous and preventable outcomes for patient safety and public health during the recent time of COVID.

AMPS – Australian Medical Professionals’ Society.

https://www.facebook.com/AMPSredunion/videos/1037024000521577

I compiled a shocking list of studies on vaccine damage here, you have not been told.

This is the real science. 

1000 ‘Under the Carpet’ Adverse mRNA Studies

Thousands of doctors, nurses, aged care, police, emergency, teachers and everyday workers in all industries have been sacked in Australia for not submitting to vaccine mandates for vaccines that clearly don’t work and do not stop transmission.

Everyone at my work got COVID and they were vaccinated, it is well past a joke.

In Brisbane when searching the job ads in Jora there are a steady 5,500 or more aged care jobs advertised, that is actually a bigger disaster in aged care since all the vaccinated workers are contracting COVID anyway, but people are left with no care at all.

I think the simple solutions to fix this problem are engineering ones where buses, trains, planes, theatres, and hospitals have the proper air control to neutralise all viruses and bacteria instead of stuffing around with vaccines that don’t work and worrying about imaginary variants.

We need to find real solutions to this stuff up, we have wasted so much money on hi-tech rubbish and ended up on the road to nowhere.

Low ozone concentration and negative ions for rapid SARS-CoV-2 inactivation

https://www.biorxiv.org/content/10.1101/2021.03.11.434968v1.full.pdf

Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses

https://www.nature.com/articles/s41598-020-67211-2

‘However, the proposed steam inhalation cycles are safe (provided care is used against the possible burns, especially in children), inexpensive and easily self-managed. This simple approach, only tested in a small sample and in a biased population, might help easing the consequences of SARS-CoV-2 infection, if applied early in at risk individuals in whatever healthcare setting, especially in low income countries with limited access to equipped hospitals or intensive care units. Unequivocal proof of efficacy would require a controlled trial on a larger scale.

Should our preliminary observations be confirmed the protocol could be used against COVID19 or other viral infections using vapotherm masks, where temperature, time of exposure and size of steam particles can be set and monitored.’

Thermal inactivation of SARS COVID-2 virus: Are steam inhalations a potential treatment?

https://www.sciencedirect.com/science/article/pii/S002432052031554X?via%3Dihub

‘By the use of a modified ionizer device we describe effective prevention of airborne transmitted influenza A (strain Panama 99) virus infection between animals and inactivation of virus (>97%). Active ionizer prevented 100% (4/4) of guinea pigs from infection. Moreover, the device effectively captured airborne transmitted calicivirus, rotavirus and influenza virus, with recovery rates up to 21% after 40 min in a 19 m3 room. The ionizer generates negative ions, rendering airborne particles/aerosol droplets negatively charged and electrostatically attracts them to a positively charged collector plate. Trapped viruses are then identified by reverse transcription quantitative real-time PCR. The device enables unique possibilities for rapid and simple removal of virus from air and offers possibilities to simultaneously identify and prevent airborne transmission of viruses.’

Ionizing air affects influenza virus infectivity and prevents airborne-transmission

https://www.nature.com/articles/srep11431

In Australia there is a saying for a poor job or a mess which applies to this, it is called a ‘dog’s breakfast’.

‘First Dog in Hong Kong
Oral and nasal samples were collected from an apparently healthy 17-year-old Pomeranian in Hong Kong that lived with a person infected with SARS-CoV-2 (the novel coronavirus that causes COVID-19). The samples tested weakly positive for the virus. The dog was quarantined, and subsequently, additional nasal and oral samples collected during the course of quarantine also tested weakly positive. This suggested that the dog may be infected, rather than just contaminated with the virus. Another dog and a cat from the same household tested negative. Subsequently, an antibody test was done on a blood sample from the dog, which initially was reported as negative, suggesting that the dog had not become infected, although there can be reasons for false negatives.

Two days after the dog was released from quarantine (March 17), it died suddenly. Unfortunately, the owner declined a postmortem. However, because it was a 17-year-old dog with advanced cardiac disease that showed no other signs during quarantine, there is a high likelihood that death was unrelated to coronavirus infection. On March 19, it was revealed that further testing done on the blood sample collected from the Pomeranian in early March showed that it was actually positive, suggesting that the dog had mounted an immune response to the virus.’

Information on Animals That Have Tested Positive for COVID-19

https://www.vetmed.ucdavis.edu/news/information-animals-covid-19

These are my other blogs on this subject.

Everyone Should Be A Whistleblower

Covid-19: Researcher blows the whistle on data integrity issues in Pfizer’s vaccine trial

https://mindhealthsoul.blogspot.com/2021/11/everyone-should-be-whistleblower.html

Why are we still using Remdesivir on COVID patients?

https://mindhealthsoul.blogspot.com/2021/11/why-are-we-still-using-remdesivir-on.html

Editorial on Vaccine Mandates (written by a US friend)

https://mindhealthsoul.blogspot.com/2021/12/editorial-on-vaccine-mandates.html

The Bizarre Silence on Pollution 2020

https://mindhealthsoul.blogspot.com/2020/10/2019-was-year-where-pollution-was-going.html

Sandstorms and the Coughing 2020

https://mindhealthsoul.blogspot.com/2020/10/sandstorms-and-coughing-2020.html

Why are we using so much Oxygen on People?

https://mindhealthsoul.blogspot.com/2020/12/why-are-we-using-so-much-oxygen-on.html

Are We Blaming The Wrong Thing? Nitrogen Dioxide Coughing  

https://mindhealthsoul.blogspot.com/2021/08/are-we-blaming-wrong-thing-nitrogen.html

Pandemics, Solar Flares, Cosmic Rays and Nitric Oxide

https://mindhealthsoul.blogspot.com/2020/05/pandemics-solar-flares-cosmic-rays-and.html